Saturday, January 23, 2016

DNA Clusters - Chromosome 1 and Chromosome 2

We uploaded the raw DNA kits for Julie, Stewart and Clinton to the website GEDMatch.  GEDMatch is a free website that allows individuals from different DNA testing companies to share their test results and collaborate.

GEDMatch also offers users many different utilities to analyze raw DNA results. One of the utilities available is a 'One-to-many' matches. The utility is run for one person and it will provide a list of kits that share DNA with that person.

Using the 'One-to-many' matches utility a list of matches was generated for Julie, Stewart and Clinton. The results were compared to identify kits shared by all three cousins. The results returned 1,500 matches per cousin. Of the 4,500 matches analyzed there were 16 DNA kits that shared DNA with Julie, Stewart and Clinton.

These 16 sets of results were then run through the utility 'One-to-one' compare. The  'One-to-one' compare allows two DNA kits to be compared. This utility will determine if two kits share DNA. If the two kits do share DNA the utility will identify the shared segments by chromosome. The utility will also show the amount of DNA shared in centiMorgans(cM) by chromosome and in total. Once the amount of shared DNA is understood a genetic table can be consulted to have a better understanding of the potential relationship between the two individuals.

The results from the 'One-to-one' compare were compiled into a spreadsheet for further analysis. After reviewing the results the majority of the DNA matches  were concentrated on Chromosome 1. The graphic below shows the 10 DNA kits that share DNA with Julie on Chromosome 1. Further review of these kits indicates there are three sub-clusters of DNA.
Michelle M. Murosky: Discovering Sally Ann &emdash;
DNA Matches on Chromosome 1
Chromosome 1 - A Sub-Cluster:
  • This cluster of DNA matches occurs on Chromosome 1. 
  • The start location is 164,433,319.
  • The end location is 173,508,458.
  • This cluster is made up of 7 DNA kits.
  • There are 4 DNA kits that match the 3 Miller/Shoup cousins (Julie, Stewart and Clinton)
  • One of the matches is in the range of a 3rd cousin to Stewart.
  • The remainder of the matches are in the 4th cousin range to all 3 Miller/Shoup cousins.
Michelle M. Murosky: Discovering Sally Ann &emdash;

Chromosome 1 - B Sub-Cluster:
  • This cluster of DNA matches occurs on Chromosome 1. 
  • The start location is 175,004,876
  • The end location is 197,583,558
  • This cluster is made up of 6 DNA kits.
  • There are 3 DNA kits that match the 3 Miller/Shoup cousins (Julie, Stewart and Clinton)
  • The  matches are in the 4th cousin range to all 3 Miller/Shoup cousins.
Michelle M. Murosky: Discovering Sally Ann &emdash;

Chromosome 1 - C Sub-Cluster:
  • This cluster of DNA matches occurs on Chromosome 1. 
  • This match has overlaps with the matches in Chromosome 1-A and Chromosome 1-B. What makes this match different is there is no break in the segment. This match is represented by the dashed lines in the overall Chromosome 1 graphic. 
  • The start location is 173,450,562
  • The end location is 198,313,067
  • This cluster is made up of 4 DNA kits.
  • There is 1 DNA kits that match the 3 Miller/Shoup cousins (Julie, Stewart and Clinton)
  • The match is in the 4th cousin range to all 3 Miller/Shoup cousins.
Michelle M. Murosky: Discovering Sally Ann &emdash;

The results also indicated there was one cousin that matched Julie, Stewart and Clinton on Chromosome 2.

Chromosome 2 - A Sub-Cluster:
  • This cluster of DNA matches occurs on Chromosome 2. 
  • The start location is 202,838,641
  • The end location is 212,302,918
  • This cluster is made up of 4 DNA kits.
  • There are 3 DNA kits that match the 3 Miller/Shoup cousins (Julie, Stewart and Clinton)
  • There is 1 DNA kits that match the 3 Miller/Shoup cousins (Julie, Stewart and Clinton)
  • The match is in the 4th cousin range to all 3 Miller/Shoup cousins.
Michelle M. Murosky: Discovering Sally Ann &emdash;

Now What? How do we use this data? 

The next step in the process will be to contact each of the matches. If the match knows their family history and is willing to share who their ancestors with us it would be very helpful to our research. These matches are all distant cousins to Julie. If we can determine how these individuals descend from Henry H. Miller and Lydia Shoup we can eliminate those descendant lines from our search.

Monday, January 18, 2016

The Henry H. Miller & Lydia Shoup DNA Cluster

Late fall of 2015 we discovered that Julie had been linked to the DNA Circle of Rhoda Osceola Elder. The DNA Circle initially indicated Julie was possibly connected to five other individuals.

Thankfully some of the individuals in the DNA Circle have Public Trees shared at Ancestry.com. A public tree means the tree is accessible to individuals with an Ancestry.com membership. We are grateful that these researchers were willing to make their research available to others.

The matches included in the DNA Circle were combined with data from the family trees to create diagram to understand how the DNA matches were connected to Henry H. Miller and Lydia Shoup.
Michelle M. Murosky: Discovering Sally Ann &emdash;
The Henry H. Miller & Lydia Shoup DNA Cluster
There are currently nine (9) individuals included in the DNA cluster. The DNA cluster contains two groups of descendants - the descendants of Lucy Ann Miller and the descendants of Henry W. Miller. There is also a grouping of descendants that link to the DNA cluster but their relationship to the cluster is currently not known.

The Descendants of Lucy Ann Miller and John Hueston Elder

Lucy Ann Miller is the daughter of Henry H. Miller and Lydia Shoup. She was born in 1842. The DNA matches linking through Lucy Ann Miller are split between two of her daughters:
  • Her daughter Pennola Lamonta Elder born in 1867 has one (1) descendant linking to the DNA cluster. 
  • Her daughter Rhoda Osceola Elder born in 1872 has six (6) descendants linking to the DNA cluster. 
The Descendants of Henry W. Miller and Emaline Weaver

Henry Washington Miller is the son of Henry H. Miller and Lydia Shoup. He was born in 1836. The descendants of Henry H. Miller are linked through his son Warren Washington Miller born in 1868.
  • His son Stewart Kenneth Miller has (1) descendant linking to the DNA cluster. 
  • His daughter Nettie Elizabeth Miller born in 1909 has one (1) descendant linking to the DNA cluster. 
The Unknown Descendants

There is a grouping of five (5) descendants that are also linked to the DNA Cluster. These individuals are linked to multiple matches in the DNA cluster but their relationship to the cluster is currently unknown.
Michelle M. Murosky: Discovering Sally Ann &emdash;
The Henry H. Miller & Lydia Shoup DNA Cluster
with Unknown Matches
The descendants with an unknown link include:
  • Julie shares DNA matches with the descendants of Pennola Lamonta Elder, Rhoda Osceola Elder and Henry W. Miller. 
  • Unknown Match 1 shares DNA with Julie and the descendants of Warren Washington Miller. 
  • Unknown Match 2 shares DNA with Julie and the descendants of Warren Washington Miller.
  • Unknown Match 3 shares DNA with a descendant of Rhoda Osceola Elder and the descendants of Warren Washington Miller. 
  • Unknown Match 4 shares DNA with Julie and possibly links to the descendants of Lucy Ann Miller. 
Making the Connection 

A matrix diagram shows that Julie shares DNA with descendants of Lucy Ann Miller and Henry W. Miller. Julie's known cousins Stewart and Clinton also share DNA with some of these cousins. The matches in the matrix below can be viewed on the DNA cluster diagram above.
Michelle M. Murosky: Discovering Sally Ann &emdash;

Using the DNA cluster and the matrix we are able to determine that Henry H. Miller and Lydia Shoup are Julie’s ancestors.

References: 
The references to descendants of Lucy Ann Miller, birth years and spouses are from Ancestry Trees.
  1. Ancestry.com Tree Number 31328622, Tree Name Victoria 
  2. Ancestry.com Tree Number 81711636, Maher Family Tree 
  3. Ancestry.com Tree Number 8198581, All the Barnes Family Tree

Sunday, January 10, 2016

Starting the Journey

Our journey started in the summer of 2014. Julie had shared on social media that she was adopted and had spent over 20 years searching for her birth family. Julie and I are related by marriage - our husbands are 1st cousins.
Michelle M. Murosky: Discovering Sally Ann &emdash;
Julie started her search on Social Media in 2014
Note that the sign contains an error. She was born it was Jamestown General Hospital
Intrigued, I reached out to her as I had been researching my family history for over 15 years. I asked Julie of she had ever considered taking a DNA test. After some discussion we ordered the AncestryDNA test for Julie.
Michelle M. Murosky: Discovering Sally Ann &emdash;
Julie with her DNA Kit
Julie completed her test in July of 2014 and the kit was sent to the test lab. While waiting for the results to come back we had an initial discussion about the unknown. We knew we would have to wait and see what types of genetic matches Julie would have and then formulate a research plan.

The initial DNA results came in late summer of 2014. Julie was very fortune to have a genetic 2nd cousin match and a handful of genetic 3rd cousin matches. The remaining matches were for more distant cousins.

An initial genetic 2nd second cousin match through DNA can be a range of possibilities. The relationship could be a 1st cousin (1x removed), a great aunt or uncle, a 2nd cousin or even a 3rd cousin.

The 2nd cousin match thankfully had a public tree shared at Ancestry.com. When initially reviewing the ages of match’s deceased parents it appeared that there was the possibility that the relationship between the match and Julie could possibly be a 1st cousin (1x removed). After an initial discussion with Julie she gave me the okay to contact the 2nd cousin on her behalf.

The First Contact 

We are very fortunate that the 2nd cousin match, Stewart, reached out to me after receiving my initial message. This was the first step in unlocking the mystery that is Julie’s heritage. Through our initial conversation Stewart was able to provide helpful details. His mother was an only child. His father, Kenneth Miller, was born in West Virginia the son of Warren Washington Miller and Nora Ida Spangler. Kenneth had two sisters Stella May Miller and Nettie Elizabeth Miller. Stella May did not have any children.

From this initial conversation we had a starting point:
  • Our initial hypothesis was that Julie was most likely connecting to Stewart through his Miller and Spangler relatives due to his mother being an only child. 
  • If the match to Stewartwas truly a 1st cousin (1x removed) – one of Julie’s parents could possibly be a descent of Nettie Elizabeth Miller. 
  • If the match was further back – Julie would be linking to S.M through the ancestors of Warren Washington Miller or Nora Ida Spangler. 
Julie and S.M. made contact in the fall of 2014. This was her very first connection with a member of her family. The two cousins continue to stay in touch.

Descendants of Nettie Elizabeth Miller 

With a starting point – the next step was to research Nettie Elizabeth Miller and see if any living descendants could be located. A timeline format was used was document the historical records associated with Warren Washington Miller and his daughter Nettie Elizabeth Miller. While researching Nettie the names of her grandsons were located. After discussing with Julie she indicated she was ready to move forward and gave me permission reach out to Nettie’s grandson, Clinton. Prior to reaching out to Clinton a letter detailing Julie’s search for her birth family and family history of Warren Washington Miller and Nora Ida Spangler. Included with the letter were ancestry charts and a copy of the Miller family timeline.

After reading the letter Clinton indicated he would be willing to speak with me. He was able to provide information about his grandmother Nettie and his extended family. Clinton also indicated he was willing to help and consented to do a DNA test. With that we ordered an AncestryDNA test for Clinton.

We were looking forward to the results of Clinton’s DNA test. After the results came in the first thing to do was to determine if Julie and Clinton were cousins. The DNA test indicated that Clinton and Stewart were genetic second cousins. From genealogy research we knew that Clinton and Stewart were 1st cousins (1x removed). The DNA test also indicated that Clinton and Julie were genetic 3rd cousins.

An initial genetic 3rd cousin match can be a range of possibilities. The relationship could be a 3rd cousin, a 2nd cousin (2x removed), a 3rd cousin (1x removed) or a 2nd (3x removed).

To better understand the potential relationship between Julie and Clinton their raw DNA kits were analyzed at GEDMatch.  GEDMatch is a free website that allows individual from different DNA testing companies to share their test results and collaborate. GEDMatch offers users many different utilities to analyze raw DNA results. One of the utilities available is a 'One-to-one' compare which allows two DNA kits to be compared. This utility will determine if two kits share DNA. If the two kits do share DNA the utility will identify the shared segments by chromosome. The utility will also show the amount of DNA shared in centiMorgans(cM) by chromosome and in total. Once the amount of shared DNA is understood a genetic table can be consulted to have a better understanding of the potential relationship between the two individuals.

Julie and Clinton share 105.4 cM of DNA across 7 chromosomes. This quantity of DNA is in the range of 2nd cousin (1x removed) and a 1st cousin (3x removed). After Clinton learned he was related to Julie the first thing he did was give her a warm welcome into the family. The cousins have remained in touch and that opportunity to meet during the summer of 2015.

From Clinton’s DNA test we knew more:
  • Julie was related to both Stewart and Clinton, this eliminated Stewart's maternal ancestors from our search. 
  • Julie and Clinton did not share enough DNA for Julie to be a descendant of Nettie Elizabeth Miller. 
  • Julie was related to the ancestors of Warren Washington Miller or she was related to ancestors of Nora Ida Spangler. 
At this point we had made progress but still didn’t have enough information to point us in a specific direction. Warren Washington Miller and Nora Ida Spangler were both a part of large families which was not uncommon in the mid to late 1800’s.

One of the challenges of identifying potential family members through genetic genealogy is patience. Progress is entirely dependent on the DNA tests completed by others. Sometimes you simply have to wait for more results to draw additional conclusions. As the popularity of DNA testing grows and more individuals get tested, the potential exists that there will be more genetic matches in time.

Possible Elimination of the Spangler Family 

After waiting several months we got another clue. In September of 2015 a woman who was researching the Spangle or Spangler family reached out. She had identified a cluster of ten cousins who were all connected to the Spangle family. These ten cousins were all linked through DNA. Clinton was sharing DNA with nine of the ten cousins in the cluster. Julie didn’t share any DNA with the cousins in the Spangler cluster.

As Julie didn’t share any DNA with the cousins in the Spangler cluster it appeared quite plausible her link to Stewart and Clinton was through Warren Washington Miller. 

A closer look at the Miller Family 

After eliminating the ancestors of Nora Ida Spangler, we were able to look closer at the Miller family to begin to establish different scenarios to determine how Julie was linking to both Stewart and Clinton. After reviewing numerous combinations of ancestor charts three scenario’s appeared the most plausible.

Scenario 1

The first scenario reviewed was the relationship between the cousins if Warren Washington Miller was the common ancestor. This analysis was built with Julie as shown as a 2nd cousin (1x removed) to Clinton. In this scenario Julie would descend from an unknown child of Warren Washington Miller.
Michelle M. Murosky: Discovering Sally Ann &emdash;

Scenario 2

The second scenario reviewed was the relationship between the cousins if Henry W. Miller, father of Warren Washington Miller was the shared ancestor. This analysis was built with Julie as shown as a 2nd cousin (1x removed) to Clinton. In this scenario Julie would descend from a sibling of Warren Washington Miller.

Michelle M. Murosky: Discovering Sally Ann &emdash;
Scenario 3

The third scenario reviewed was the relationship between the cousins if Henry H. Miller, father of Henry W. Miller, father Warren Washington Miller was the shared ancestor. This analysis was built with Julie as shown as a 3rd cousin to Stewart. In this scenario Julie would descend from a sibling of Henry W. Miller.

Michelle M. Murosky: Discovering Sally Ann &emdash;
Scenario 4

The fourth scenario reviewed was the relationship between the cousins if an ancestor of Emaline Weaver, mother of Warren Washington Miller was the shared ancestor. This analysis was built with Julie as shown as a 3rd cousin to Stewart. In this scenario Julie would descend from a sibling of Emaline Weaver.

Michelle M. Murosky: Discovering Sally Ann &emdash;
After reviewing the four scenarios we still didn’t have quite enough information to determine which family we should start researching in more detail.

Link to the DNA Circle of Rhoda Osceola Elder 

Periodically we would check Julie’s DNA results on both AncestryDNA and GEDMatch to look for additional close cousin matches. Late fall of 2015 we discovered that Julie had been linked to the DNA Circle of Rhoda Osceola Elder. The DNA Circle indicated Julie was possibly connected to five other individuals.

Thankfully some of the individuals in the DNA Circle had public trees available on Ancestry.com. Through these trees we learned that Rhoda Osceola Elder is the daughter of Lucy Ann Miller and the granddaughter of Henry H. Miller and Lydia Shoup. Four of the individuals were descendants of Rhoda Osceola Elder. Three of the individuals in the circle were sharing DNA with Julie. Five of the individuals in the circle were sharing DNA with Stewart. Two of the individuals in the circle were sharing DNA with Clinton.

Using this information from the DNA Circle we were able to identify an additional cousin that was also a descendant of Henry H. Miller and Lydia Shoup that shares DNA with Julie but does not share SNA with S.M. or Clinton.

The majority of these cousin matches were more distant. One was in the 4th – 6th cousin range and the others in the 5th – 8th cousin range to Julie.

From this cluster of Miller cousins we learned:
  • Confirmation that Julie is a Miller descendant through the connections to multiple cousins. 
  • The common ancestors that currently link all of the cousins are Henry H. Miller and Lydia Shoup. 
  • The current analysis indicates Julie shares DNA with descendants of Lucy Ann Miller and Henry W. Miller, two of the nine children of Henry H. Miller and Lydia Shoup. 
At this time there is enough evidence to indicate that Julie is a descendant of Henry H. Miller and Lydia Shoup. The next step in the journey will to learn more about Henry H. Miller and Lydia Shoup and attempt to determine how she ties into the family.